il-4 cytokine Search Results


recombinant mouse il 1β  (MedChemExpress)
94
MedChemExpress recombinant mouse il 1β
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Recombinant Mouse Il 1β, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/Animal-Free+IL-24%2C+Mouse/pmc12809408-197-26-31
Average 94 stars, based on 1 article reviews
recombinant mouse il 1β - by Bioz Stars, 2026-10
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recombinant follicle stimulating hormone  (Rockland Immunochemicals)
90
Rockland Immunochemicals recombinant follicle stimulating hormone
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Recombinant Follicle Stimulating Hormone, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/IL-4+Human+Recombinant+Protein/pm27112701-51-6-14
Average 90 stars, based on 1 article reviews
recombinant follicle stimulating hormone - by Bioz Stars, 2026-10
90/100 stars
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il-4 cytokine  (Nippo Corporation)
90
Nippo Corporation il-4 cytokine
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 4 Cytokine, supplied by Nippo Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pmc03217977-80-34-31
Average 90 stars, based on 1 article reviews
il-4 cytokine - by Bioz Stars, 2026-10
90/100 stars
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il-4 cytokine  (ImmunoTools)
90
ImmunoTools il-4 cytokine
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 4 Cytokine, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pm24465735-63-5-9
Average 90 stars, based on 1 article reviews
il-4 cytokine - by Bioz Stars, 2026-10
90/100 stars
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il-4 cytokine  (Collaborative Research Inc)
90
Collaborative Research Inc il-4 cytokine
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 4 Cytokine, supplied by Collaborative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pmc03700537-95-0-4
Average 90 stars, based on 1 article reviews
il-4 cytokine - by Bioz Stars, 2026-10
90/100 stars
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human recombinant il-4  (GENTAUR Inc)
90
GENTAUR Inc human recombinant il-4
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Human Recombinant Il 4, supplied by GENTAUR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pm30605776-37-10-13
Average 90 stars, based on 1 article reviews
human recombinant il-4 - by Bioz Stars, 2026-10
90/100 stars
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interleukin-4 (il-4)  (CreaGene Inc)
90
CreaGene Inc interleukin-4 (il-4)
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Interleukin 4 (Il 4), supplied by CreaGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pm39330237-105-33-39
Average 90 stars, based on 1 article reviews
interleukin-4 (il-4) - by Bioz Stars, 2026-10
90/100 stars
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il-17a cytokine  (Feto Maternal and GenetYX Center)
90
Feto Maternal and GenetYX Center il-17a cytokine
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 17a Cytokine, supplied by Feto Maternal and GenetYX Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pmc08595633-278-4-8
Average 90 stars, based on 1 article reviews
il-17a cytokine - by Bioz Stars, 2026-10
90/100 stars
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il-4 cytokines  (Becton Dickinson)
90
Becton Dickinson il-4 cytokines
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 4 Cytokines, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/pmc06239199-248-23-21
Average 90 stars, based on 1 article reviews
il-4 cytokines - by Bioz Stars, 2026-10
90/100 stars
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il4 (10 ng/ml, bioabchem ladson, sc)  (BioAbChem Inc)
90
BioAbChem Inc il4 (10 ng/ml, bioabchem ladson, sc)
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il4 (10 Ng/Ml, Bioabchem Ladson, Sc), supplied by BioAbChem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il4+cytokine/10__1074_slash_jbc__m115__702092-54-8-11
Average 90 stars, based on 1 article reviews
il4 (10 ng/ml, bioabchem ladson, sc) - by Bioz Stars, 2026-10
90/100 stars
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il-2 cytokine  (Immunex Corporation)
90
Immunex Corporation il-2 cytokine
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 2 Cytokine, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/10__1128_slash_iai__69__9__5270___5277__2001-49-4-14
Average 90 stars, based on 1 article reviews
il-2 cytokine - by Bioz Stars, 2026-10
90/100 stars
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il-4  (Schering-Plough corporation)
90
Schering-Plough corporation il-4
Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) <t>after</t> <t>IL-1β</t> treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.
Il 4, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il-4+cytokine/il+4+cytokine/us08722855-2010-7-10
Average 90 stars, based on 1 article reviews
il-4 - by Bioz Stars, 2026-10
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Image Search Results


Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) after IL-1β treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.

Journal: Computational and Structural Biotechnology Journal

Article Title: seneR: An R package for comprehensive senescence assessment and its application in type 2 diabetes and osteoarthritis

doi: 10.1016/j.csbj.2025.12.031

Figure Lengend Snippet: Verification of the therapeutic effect of PM in delaying the senescence of chondrocytes. (a) Schematic diagram of primary chondrocyte isolation and in vitro experimental design. (b) Representative images of brightfield, Alcian blue, Safranin O, and Toluidine blue staining of chondrocytes. (c, d) Protein levels of p16 and SLPI detected by Western blot (WB) after IL-1β treatment (c) or Slpi overexpression (OE-Slpi; d). (e, f) Representative images of SA-β-gal staining (e) and quantitative analysis of positive cells (f) in OE-negative control (OE-NC) or OE-Slpi chondrocytes. (g, h) Representative images of SA-β-gal staining (g) and quantitative analysis of positive cells (h) in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (i, j) Quantitative real-time PCR (qPCR) analysis of p16 and MMP3 mRNA expression in chondrocytes treated with 10 ng/ml IL-1β alone or combined with 1 μM PM. (k, l) Representative images of SA-β-gal staining (k) and quantitative analysis of positive cells (l) in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. (m, n) qPCR analysis of p16 and MMP3 mRNA expression in chondrocytes treated with H₂O₂ alone or combined with 1 μM PM. Data are presented as mean ± standard deviation (n = 3 independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (Student’s t -test: f; ANOVA: h, i, j, l, m, n). OE, overexpression; NC, negative control.

Article Snippet: For over-expression SLPI, chondrocytes were transfected with pcDNA3.1/Slpi full-length plasmid using Lipo8000 (Beyotime) according to the manufacturer’s instructions.For IL-1β treatment, mouse primary chondrocytes were treated with recombinant mouse IL-1β (10 ng/ml, MedChemExpress) for 24 h. For H2O2-induced senescence, mouse primary chondrocytes were treated with H2O2 (200 μM, MKBio Shanghai) for 2 h. For PM treatment, mouse primary chondrocytes were treated with or without PM (MedChemExpress), dissolved in DMSO (Dimethyl sulfoxide, MedChemExpress).

Techniques: Isolation, In Vitro, Staining, Western Blot, Over Expression, Negative Control, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation